Use the ladder for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly. Includes 11 protein bands from 8 to 260 kDa (see figure). No conjugate is needed for fluorescent blot detection. The Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection. Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3, 0.01% bromophenol blue.įluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. Packaging Options Number of Applications (Volume) In western blots, the extent of USP knockdown was quantified by running serial dilutions to the level of 50 (lane 50),75 (lane 75) and 100 (lane 100), of accessory gland proteins from ten. Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range.The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat. Molecular weight sizing of proteins after blot development Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting.Artifacts from overloading the protein marker or ladder: Load less of the molecular weight marker onto the gel. Select protein standards that offer: Good resolution of the proteins in the size range of interest. Change to membrane with smaller pore size. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Monitoring blot transfer efficiency with easy orientation via multicolor standards Solve your western blot problems with this western blot troubleshooting guide, covering high background. Western blotting protein standards can be used for both fluorescent visualization and colormetric or chemiluminescent immunodetection on western blots.Chameleon 700 or Chameleon 800 protein ladders can be used for one-channel analysis. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development. Ensure good size coverage with Chameleon ladders ranging from 8 kDa to 260 kDa Easily visualize gel migration and protein size Orient your gel or membrane quickly Chameleon Pre-stained Protein Ladders are optimized for use with Bis-Tris and Tris-Glycine gels. Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE.
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